Composite

Part:BBa_M31530:Design

Designed by: Matt Gethers   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-28)


Refactored Portion of M13K07 Genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3251
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to organize the M13K07 genome into individual components, some sequences must be "copied and pasted" elsewhere. This opens the possibility for recomination events or coding regions that code for functions for which they are no longer responsible (legacy functions). These interactions must be addressed to avoid interactions bewteen stretches of DNA. As far as I know, this can be addressed by changing codon usage, introducing inhibitory secondary structures to the DNA, or by eliminating the enzymatic mechanisms by which these events may occur. I'm sure there are others. Another consideration is that absolute and relative position in the genome may be of importance. I will try to maintain the "localities" as best I can so as not to introduce further sources of problems.


Source

This part does not yet exist in a physical form, though it may be synthesized in the near future.

References